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| 产品名称： | 639-V |
|---|---|
| 商品货号： | TS259572 |
| <strong>Cell line </strong>： | 639-V |
| DSMZ no. ： | TS259572 |
| Species ： | confirmed originally as human with IEF of AST, MDH later with STR typing |
| Cell type ： | ureter transitional cell carcinoma |
| Origin ： | established from the transitional cell carcinoma (small, grade III) removed from the left ureter of a 69-year-old white man in 1973 |
| Reference(s) ： | 14506, 14505 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherently in monolayers; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/25 cm2; split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 6 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 24% polyploidy - 55(50-59)<2n>XX, +1, +3, +5, +6, +8, +11, +12, +14, -15, +20, -21, add(1)(p?21), i(5p), del(6)(q13-14), i(7p), add(8)(p2?), add(11)(p11), del(12)(q21q24), add(18)(q23) - additional copies of 6q-, i(7p), 8p+, der(18) markers present in sidelines together with del(7)(p13-14) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |