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| 产品名称： | 647-V |
|---|---|
| 商品货号： | TS259573 |
| <strong>Cell line </strong>： | 647-V |
| DSMZ no. ： | TS259573 |
| Species ： | confirmed originally as human with IEF of AST, NP and later by STR-typing |
| Cell type ： | urothelial bladder carcinoma |
| Origin ： | established from a male patient with a primary urothelial bladder carcinoma (malignant, grade 2) |
| Reference(s) ： | 14505 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing in monolayers |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:4 to 1:8 every 3-4 days using trypsin/EDTA (wash twice with PBS before trypsination) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid/hypotriploid karyotype with 12% polyploidy - 52-60<3n>XX, -X/Y, -2, -4, -6, -9, -10, -12, -12, -14, -15, -16, -17, -18, +5mar, del(1)(p32), del(2)(q23)/add(2)(q2?), add(3)(q27), add(3)(q1?), i(5p), i(6p), i(7p)x2, add(8)(q24), del(11)(q22q24), del(13)(q?11q?14), der(19)t(12;19)(q13;q13), add(21)(p11) - i(5p) associated with bladder cancer |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |