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| 产品名称： | MFE-280 |
|---|---|
| 商品货号： | TS259575 |
| <strong>Cell line </strong>： | MFE-280 |
| DSMZ no. ： | TS259575 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | endometrium adenocarcinoma |
| Origin ： | established from tumor tissue from a 77-year-old woman with recurrence of endometrial carcinoma (adenomatous, partly papillary, grade G3) in 1990; described as forming heterotransplantable tumors in nude mice and as carrying receptors for progesterone |
| Reference(s) ： | 14587, 14588 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 40% RPMI 1640 + 40% MEM (with Earles salts) + 20% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture ： | split confluent culture 1:2 to 1:6 every 3-5 days using trypsin/EDTA; seed out at 1-3 x 106 cells/25 cm2; about 4 hours after thawing, floating cells and medium should be decanted into a second flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-90 hours |
| Harvest ： | cell harvest of ca. 2-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 24% polyploidy - 52(48-52)<2n>X, -X, +1, +1, -2, +9, -10, -12, -13, -16, -17, -17, -18, -19, -20, -22, +18mar, add(1)(p34), add(1)(p3?), add(1)(q11), add(3)(q13), add(3)(q27-29), add(4)(p14), der(9)t(9;13)(p11;q11), add(15)(p11) - near diploidy and gain of 1q typical in endometrial carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |