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| 产品名称： | MKN-45 |
|---|---|
| 商品货号： | TS259577 |
| <strong>Cell line </strong>： | MKN-45 |
| DSMZ no. ： | TS259577 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | gastric adenocarcinoma |
| Origin ： | established from the poorly differentiated adenocarcinoma of the stomach (medullary type) of a 62-year-old woman |
| Reference(s) ： | 14790, 14795, 14886 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells can be difficult to culture; seed out at ca. 1-2 x 106 cells/80 cm2; split semi-confluent culture 1:3 to 1:5 every 5-10 days using trypsin/EDTA (for 5 min or longer); culture medium may turn acidic (yellow) very quickly, exchange of spent medium is advised, also the cells in suspension may be used to seed a new flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 70-73<3n>XX/XXX, -X, -2, -2, -4, -5, -5, +6, -11, -13, -13, -14, -18, -19, -20, -21, -22, +20mar, add(X)(q1?), der(1)(1pter->p21::1p11->q32::1q44->q11::?), i(2p), der(3)t(3;?9)(p14;q13/21), del(4)(q21), del(4)(q26), del(6)(q1?)x2, del(7)(p11), del(8)(p12), add(8)(q24), del(9)(p11), add(13)(p11), inv(16)(p13q22), add(17)(p1?), add(19)(q13), i(21q) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |