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| 产品名称： | RT-112 |
|---|---|
| 商品货号： | TS259578 |
| <strong>Cell line </strong>： | RT-112 |
| DSMZ no. ： | TS259578 |
| Species ： | confirmed originally as human with IEF of LDH, NP |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the transitional cell carcinoma (histological grade G2) excised from a woman (age unknown) with untreated primary urinary bladder carcinoma in 1973; described in the literature to form tumors in nude mice |
| Reference(s) ： | 14738, 14734 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayer; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium with initially 20% FBS; split culture prior to confluence 1:2 to 1:4 every 2-5 days using extended trypsin/EDTA treatment (5-10 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 20-30 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 47(44-47)<2n>XX/XXX, +X, +10, +11, +14, -17, +mar, add(2)(q3?2), del(3)(p14p24.1), i(4p), add(6)(q2?), i(8q), del(9)(p21p22), add(10)(p12), der(11)t(3;11)(q11-13;p15), add(17)(p1?1), add(18)(q11-22) - sideline with: idem, del(9)(p11), del(11)(q23), add(12)(q24.32), del(13)(q11q14), add(21)(p11) - resembles published karyotypes |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |