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| 产品名称： | HN |
|---|---|
| 商品货号： | TS259580 |
| <strong>Cell line </strong>： | HN |
| DSMZ no. ： | TS259580 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | oral squamous cell carcinoma |
| Origin ： | established from the tumor of a 60-year-old Japanese man with moderately differentiated squamous cell carcinoma of the soft palate which was invasive into muscle layer; cell line was established from a cervical lymph node metastasis which occurred seven years after initial treatment of the primary tumor; the tumor metastasized not only to the lungs, but also to the brain; described as anchorage-independent and tumorigenic in nude mice |
| Reference(s) ： | 14652 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogeneous) growing in multilayers |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 every 5 days using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2 in 15 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 8-10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 4% polyploidy - 60-64<3n>XX, -Y, +1, -2, -4, +5, -6, +7, -10, -12, -13, -13, -17, -18, -18, -21, -22, +3-5mar, add(X)(p22), der(1)add(1)(p36)ins(1;?)(q11;?), t(4;14)(q21;p11), i(4p)x1-2, del(5)(q31), i(5p), add(6)(p23), del(6)(q15), add(7)(q31), der(8)t(8;11)(p11;p11)add(8)(q24), add(11)(q11), add(11)(q24), ins(12;?)(q21;?)dup(12)(q11q24.32), dup(13)(q11q32), der(14)t(8;14)(q11;p11), i(14q), add(15)(p11), add(15)(p11), add(?15)(p11), der(17)t(13;17)(q13;q22), add(19)(p13) - sideline with del(1)(p11), del(7)(q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |