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| 产品名称： | C7 |
|---|---|
| 商品货号： | TS259581 |
| <strong>Cell line </strong>： | C7 |
| DSMZ no. ： | TS259581 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | macrophages |
| Origin ： | established from the bone marrow of male adult 129/Sv mice (p53 gene knocked out); it is described that the differentiation potential of C7 cells to osteoclasts is maintained in the presence of M-CSF and is abolished irreversibly by the stimuli known to induce differentiation into other hematopoietic lineages |
| Reference(s) ： | 14771 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | macrophage-like adherent cells with processes |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 1 ng/ml M-CSF (human or murine) |
| Subculture ： | split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA after washing twice with PBS; seed out at ca. 0.5 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of ca. 2-3 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype with 20% polyploidy - 66(64-67)<3n> - no centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |