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| 产品名称： | RLD-1 |
|---|---|
| 商品货号： | TS259583 |
| <strong>Cell line </strong>： | RLD-1 |
| DSMZ no. ： | TS259583 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and with species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | T cell leukemia |
| Origin ： | established from transplantable tumors induced in syngeneic mice inoculated with cell suspensions prepared from lymph nodes infiltrated by lymphatic leukemia induced in 6-week-old DBA/2 mice by Rauscher virus (present in supernate of JLSV-9 cells); described as expressing T cell surface markers (Thy-1, T-200, Lyt-1), high constitutive TdT activity, and as differentiating along T-helper lineage in response to treatment with DMSO or phorbol ester TPA |
| Reference(s) ： | 14451 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroidal cells growing singly in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; optimal split ratio of 1:2 to 1:6 every 2-4 days; cell growth may be slow for the first few days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-30 hours |
| Harvest ： | saturation density of ca. 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hyperdiploid karyotype - 42<2n>XX, +14, +15 - no centric fusion markers present - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |