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| 产品名称： | MFE-296 |
|---|---|
| 商品货号： | TS259587 |
| <strong>Cell line </strong>： | MFE-296 |
| DSMZ no. ： | TS259587 |
| Species ： | confirmed originally as human with IEF of LDH, NP and later by STR fingeprinting |
| Cell type ： | endometrium adenocarcinoma |
| Origin ： | established from the primary tumor of a 68-year-old Caucasian woman with moderately differentiated endometrial adenocarcinoma (grade G2) in 1991; cells were described in the literature to express androgen receptors (androgen was reported to inhibit cell proliferation), but no estrogen receptors; cells were described in the literature to be tumorigenic in nude mice |
| Reference(s) ： | 14586, 14587 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like polymorphic cells growing adherently in monolayers |
| Medium ： | 40% RPMI 1640 + 40% MEM (with Earlesalts) + 20% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human diploid karyotype with 20% polyploidy - 46<2n>XX, no consistent abnormality detected - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/I I-, MLV -, SMRV - |