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| 产品名称： | 1G1 |
|---|---|
| 商品货号： | TS259590 |
| <strong>Cell line </strong>： | 1G1 |
| DSMZ no. ： | TS259590 |
| Species ： | originall confirmed as mouse with IEF of AST, LDH, NP and with species PCR and recently as mus musculus by COI-DNA Barcoding |
| Cell type ： | hybridoma (anti-chicken dorsal retina cytoplasmic membranes, E12) |
| Origin ： | cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina (inclusive chick, mouse, rat) |
| Reference(s) ： | 14941 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroidal cells growing singly in suspension, or occasionally loosely adherent |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | maintain at ca. 0.1-0.3 x 106 cells/ml; split saturated culture 1:3 to 1:10 every 2-5 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.3 x 106 cells/ml; a maximal density of 1.0 x 106 cells/ml should not be exceeded |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype - 94-102<4n> - no centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |