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| 产品名称： | MFE-319 |
|---|---|
| 商品货号： | TS259592 |
| <strong>Cell line </strong>： | MFE-319 |
| DSMZ no. ： | TS259592 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | endometrium adenocarcinoma |
| Origin ： | established from the primary adenosquamous endometrium carcinoma (grading G1-G2) of an 81-year-old woman in 1992; described in the literature to form poorly differentiated tumors in nude mice |
| Reference(s) ： | 14587 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayer; image; image |
| Medium ： | 40% RPMI 1640 + 40% MEM (with Earles salts) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 5 days using trypsin/EDTA (cells are difficult to detach from the flask; incubation with trypsin/EDTA of 15 min or longer at 37 °C is recommended); maximum density of ca. 20 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-90 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 4% polyploidy - 45(43-46)<2n>X, -X, t(13;18)(q14.3-21;q11-12) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |