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| 产品名称： | MFM-223 |
|---|---|
| 商品货号： | TS259593 |
| <strong>Cell line </strong>： | MFM-223 |
| DSMZ no. ： | TS259593 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of a post-menopausal breast cancer patient without any prior treatment (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described in the literature to express androgen, estrogen and progesterone receptors |
| Reference(s) ： | 14585 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing as monolayer with reticulate growth pattern; image; image |
| Medium ： | 85% MEM (with Earles salts) + 15% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture ： | cells grow very slowly; split confluent culture 1:2 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2 in 10 ml medium |
| Incubation ： | at 37 °C without CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 5-7 days |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(7)(q31-32), add(8)(p22-23), add(8)(q?24), del(10)(q2?), add(11)(q22), dup(12)(q13q14), add(13)(p11), add(15)(p11), add(17)(p1?), add)19)(q13), add(21)(p11), add(?22)(q13) - carries multiple hsr including ch 7 material - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |