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| 产品名称： | SER-W3 |
|---|---|
| 商品货号： | TS259595 |
| <strong>Cell line </strong>： | SER-W3 |
| DSMZ no. ： | TS259595 |
| Species ： | confirmed as rat with IEF of AST, LDH, NP and with species PCR |
| Cell type ： | rat Sertoli cells |
| Origin ： | Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion) |
| Reference(s) ： | 14855 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing in monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split 90% confluent culture 1:2 to 1:5 every 2-6 days using trypsin/EDTA; seed out at ca. 0.3-0.5 x 105 cells/cm2; maintain at ca. 0.1-0.3 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 0.8 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |