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| 产品名称： | KG-1a |
|---|---|
| 商品货号： | TS259596 |
| <strong>Cell line </strong>： | KG-1a |
| DSMZ no. ： | TS259596 |
| Species ： | confirmed originally as human with IEF of LDH, NP and later by STR fingerprinting |
| Cell type ： | acute myeloid leukemia (derivative of KG-1) |
| Origin ： | established from the bone marrow of a 59-year-old man with erythroleukemia that developed into acute myeloid leukemia (AML); KG-1a is a subclone of AML cell line KG-1 (DSM ACC 14) described as "undifferentiated variant" |
| Reference(s) ： | 14679 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly single round or 1-2% giant cells growing in suspension, some cells are loosely adherent; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) or RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1.0 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | maximum density at ca. 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 +; image |
| Fingerprint ： | same DNA profile as KG-1 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near-diploid karyotype with 5% polyploidy - 46(44-47)<2n>X/XY, +8, +11, -12, -17, -20, der(5;17)(q10;q10)del(5)(q?11q?13), del(7)(q22q35), i(8q), der(8)t(8;12)(p11;q13)add(8)(q24), i(11q), add(16)(q13/21), der(19)t(14;19)(q11;q13), add(20)(p13) - sideline with -18 - resembles published karyotype - closely related to, but distinct from KG-1 |
| Molec. Genetics ： | expression of fusion gene FGFR1OP2-FGFR1 (OP2-FGFR1) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |