微信小李
微信小章
产品简介
购买须知
| 产品名称： | VLM |
|---|---|
| 商品货号： | TS259602 |
| <strong>Cell line </strong>： | VLM |
| DSMZ no. ： | TS259602 |
| Species ： | originally confirmed as mouse with IEF of AST, NP by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | SV40-transformed embryo cells |
| Origin ： | established from SV40-transformed BALB/c mouse embryo cells; cells are described as possessing SV40 T-antigen and tumor-specific transplantation antigens and as not releasing spontaneously SV40; tumorigenic in mice |
| Reference(s) ： | 15075 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing in monolayers |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA; seed out at ca. 2-3 x 105 cells/ml in 10 ml medium; |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | cell harvest of maximal 13 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |