微信小李
微信小章
产品简介
购买须知
| 产品名称： | D3 |
|---|---|
| 商品货号： | TS259604 |
| <strong>Cell line </strong>： | D3 |
| DSMZ no. ： | TS259604 |
| Species ： | originally confirmed as murine with IEF of LDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-chicken N-CAM) |
| Origin ： | cell line was established by fusion of mouse NS-1 cells with spleen cells from a BALB/c mouse immunized with wheat germ agglutinin affinity purified chicken brain proteins immobilized on lectin beads; described as secreting a mouse monoclonal antibody (IgG) reacting with the cytoplasmic domain of the 140, 180 and 250 kDa glycosylated neuronal cell surface protein N-CAM, preferentially in neurons of the chicken visual system like retina, tectum and other CNS regions and PNS; also reacting with mouse, goldfish and human N-CAM in paraformaldehyde-fixed cryosections and Western blots |
| Reference(s) ： | 14937 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension and some slightly adherent |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") |
| Subculture ： | maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:5 to 1:10 every 2-3 days (some cells attach to the surface of the culture flask, but can be detached by tapping the flask); seed out at ca. 0.5 x 106 cells/ml; maximum density of ca. 1-2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 22 hours |
| Harvest ： | cell harvest of ca. 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2A monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype - 98-104<4n> - two centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |