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| 产品名称： | DU-4475 |
|---|---|
| 商品货号： | TS259605 |
| <strong>Cell line </strong>： | DU-4475 |
| DSMZ no. ： | TS259605 |
| Species ： | confirmed as human with IEF of AST, MDH, NP (loss of LDH isoenzymes) |
| Cell type ： | breast carcinoma |
| Origin ： | established from the local metastatic cutaneous nodule of an undifferentiated carcinoma of a 62-year-old woman which developped seven years after radical mastectomy of a poorly differentiated ductal carcinoma of the breast; cells were described in the literature to grow readily in athymic nude mice and to produce tumors from which cells could be introduced into culture in vitro; cells reportedly express components of milk fat globules and the BCL1 gene |
| Reference(s) ： | 14529, 14704 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly or in clusters in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 once a week; seed out at ca. 0.2 x 106 cells/ml; maintain at ca. 0.2 x 106 cells/ml; maximum cell density at ca. 0.5 x 106/ml; due to cluster formation, cells are difficult to count; cultures may contain large amounts of debris and viability may be consistently below 70% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of ca. 0.3-0.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-tetraploid karyotype with 12% polyploidy - 88-93<4n>XXXX, +1, +1, -5, -6, +9, -10, -10, +15, +15, -16, -16, +22, +4mar, i(1q)x2, ?add(1)(p35-36)x2, ?i(5p)x2, add(6)(p11), add(6)(p1?), del(6)(q25), add(9)(q35), del(11)(q24)x2, add(15)(p11)x2, add(17)(p1?)x2, del(21)(q22.2)x2 - sideline with -20, -20, +del(7)(p11) - gain of 1q and loss of 6q typical in breast carcinoma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |