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| 产品名称： | 8-MG-BA |
|---|---|
| 商品货号： | TS259613 |
| <strong>Cell line </strong>： | 8-MG-BA |
| DSMZ no. ： | TS259613 |
| Species ： | originally confirmed as human with IEF of AST, LDH and later by STR fingerprinting |
| Cell type ： | glioma |
| Origin ： | established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment) |
| Reference(s) ： | 14846 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like, adherent cells growing as monolayer |
| Medium ： | 80-85% MEM (with Earles salts) + 15-20% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:4 once a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2; optimal density at ca. 5-10 x 106/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of maximal 15 x 106/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8q), add(8)(p21)x1-2, del(9)(q32q34)/add(9)(q32), add(11)(?q13), del(12)(q14), del(18)(q22.2), add(19)(p13.1) - sideline with del(3)(p11), der(6;9)(p10;q10) - 2-4 acf present in 31% cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |