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| 产品名称： | SK-MM-2 |
|---|---|
| 商品货号： | TS259616 |
| <strong>Cell line </strong>： | SK-MM-2 |
| DSMZ no. ： | TS259616 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH, NP |
| Cell type ： | plasma cell leukemia |
| Origin ： | established from the peripheral blood of a 54-year-old man with plasma cell leukemia (Igkappa) (refractory, terminal state) in 1982; plasma cell leukemia is related to multiple myeloma |
| Reference(s) ： | 14486, 14521, 14809 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round single cells growing in suspension, cells may contain microvesicles; image ; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells are difficult to culture; seed out at ca. 1.0 x 106 cells/ml; cells grow slowly during the first week after recovery from liquid nitrogen; do not culture below 0.6 x 106 cells/ml; split saturated culture 1:2 every 3-4 days; maximum density of ca. 1.3 x 106 cells/ml; long-term cultivation might lead to accumulation of exosomes or microvesicles, however without affecting viability and proliferation |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100 hours |
| Harvest ： | cell harvest of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hypodiploid/hypotetraploid karyotype with 9% polyploidy (including a subpopulation with 32-64N) - 42(38-43)<2n>XX, -13, -18, -22, del(1)(p32p36), der(2)t(1;2)(p32;q3?1)t(1;13)(p36q1?4), der(4;14)(4pter->4p15::4p11->4p10::14q10->14q22::4q11-12->4qter), del(6)(q15), i(8q), der(14)t(11;14)(q13;q32) (46%) - 84(78-84)<4n>idemx2 (45%) - carries non-reciprocal t(11;14) causing rearrangement of CCND1 (BCL1) associated with chronic lymphoproliferative disorders - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |