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| 产品名称： | MOLT-14 |
|---|---|
| 商品货号： | TS259621 |
| <strong>Cell line </strong>： | MOLT-14 |
| DSMZ no. ： | TS259621 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from the bone marrow of a 2-year-old girl with T-ALL (T cell acute lymphoblastic leukemia, FAB L2) at relapse in 1983; MOLT-13 (DSM ACC 436) is a simultaneous sister cell line derived from the same patient sample with similar or identical features |
| Reference(s) ： | 14477 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split saturated culture 1:4 to 1:5 twice a week; culture medium becomes rarely acidic (yellow) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 +, CD5 +, CD7 +, CD8 -, CD13 -, CD19 -; image |
| Fingerprint ： | same DNA profile as MOLT-13 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human pseudodiploid karyotype with 8% polyploidy - 46(44-48)<2n>XX, der(17)t(17;17)(p13;q22.1) - resembles published karyotype - karyotypically identical to MOLT-13 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |