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| 产品名称： | EVSA-T |
|---|---|
| 商品货号： | TS259625 |
| <strong>Cell line </strong>： | EVSA-T |
| DSMZ no. ： | TS259625 |
| Species ： | confirmed originally as human with IEF of AST, MDH, NP and later by STR fingerprinting |
| Cell type ： | breast carcinoma |
| Origin ： | established from the malignant ascitic effusion from a woman with metastatic breast carcinoma; described as being estrogen receptor-negative and progesterone receptor-positive |
| Reference(s) ： | 14400, 14718, 14447, 14702, 15191 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 2-5 x 106 cells/80 cm2; split semi-confluent culture 1:3 every 3-4 days using trypsin/EDTA, dissociate cells with a pipette (10 ml) during trypsination; after thawing and in the first week, viability and adherence may be low (about 50%) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of about 20-30 x 106 per 80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 4% polyploidy - 62(55-64)<3n>XXX, +1, -2, -5, -5, +6, -8, -9, -10, -13, -13, -14, -15, -16, -17, -18, -20, -22, +6-8mar, add(X)(p11), der(X)t(X;9)(q21;q11), add(1)(p1?1), der(1)del(1)(p36)dup(1)(q22q32), del(1)(q11), der(1;4)(q10;q10)del(4)(q1?13q?23), der(2)t(2;5)(p11;q13)add(2)(q3?5), der(3)t(3;5)(p14;q11), del(4)(q22), del(6)(q25q27), del(6)(p11), add(6)(p23), del(7)(p14), add(8)(q24), ider(8)(q10)ins(8;?)dup(8)(?q21q24)add(8)(q24), add(11)(p15), add(12)(p13), del(12)(p13), dup(13)(q21q33), add(21)(p11), add(21)(p11), large submetacentric der(8) hsr marker present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |