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| 产品名称： | CAL-85-1 |
|---|---|
| 商品货号： | TS259629 |
| <strong>Cell line </strong>： | CAL-85-1 |
| DSMZ no. ： | TS259629 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast adenocarcinoma |
| Origin ： | established from the relapsing invasive galactophoric breast adenocarcinoma resected from a 35-year-old woman during radiotherapy in 1990; cells were described as expressing the multidrug resistance (MDR) gene |
| Reference(s) ： | 15134, 15199 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/175cm2; split confluent culture 1:2 to 1:4 every 3-4 days using trypsin/EDTA (incubation for about 10 min); a significant amount of cell debris is always visible in the culture background which might be due to granula or other intracellular material |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of 10-15 x 106 cells/175cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 8% polyploidy - 60(57-65)<3n>XX/XXX, +2, +5, -8, -10, -12, -13, -14, -15, -16, -16, -17, -18, +?20, -21, -22, +2-4mar, +fra, add(X)(q2?), del(X)(q11), add(1)(p34), del(2)(q?11q?21)x1-2, add(2)(p2?3), der(3)?inv(3)(p24q29)add(3)(qter), der(3)del(3)(p13)dup(3)(?q13?q26)del(3)(q26qter), del(3)(q21), add(4)(q2?9), der(4)t(4;9)(p15;?q22), der(6)t(6;12)(q22-24;q13), add(7)(p11), del(7)(q32)x2, der(9)dup(9)(p11p24)add(9)(p24), add(11)(q24-25)x1-2, del(12)(p13), der(15;21)(q10;q10), add(17)(p13), add(17)(p13), add(19)(p13), der(?20)t(17;20)(q12;q13)dup(17)(q1?2q2?3), add(21)(p11) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |