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| 产品名称： | CAL-72 |
|---|---|
| 商品货号： | TS259630 |
| <strong>Cell line </strong>： | CAL-72 |
| DSMZ no. ： | TS259630 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | osteosarcoma |
| Origin ： | established from a chemotherapy-resistant tumor sample obtained from the left knee of a 10-year-old boy with anaplastic osteoblastic sarcoma (Rosen grade IIB) in 1989 |
| Reference(s) ： | 14896 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid cells growing adherently in monolayers; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human, bimodal hyperdiploid/hypertetraploid karyotype with 12% polyploidy - 45-56<2n>X, -Y, +1, +1, -2, -2, +8, +11, -13, -15, +19, -20, -20, -22, -22, +15-20mar, +dmin, add(X)(q21), der(1;19)(q10;q10), add(1)(p3?5)add(1)(q3?), add(3)(q2?9)x2, add(6)(q11), del(7)(q11), add(8)(q24), add(8)(q11), add(11)(p1?), del(11)(q13), add(14)(q3?)x2, add(16)(p11), add(16)(q23), add(19)(p11), add(21)(p11) (hyperdiploid clone) - additional rearrangements, including der(1;8)(q10;q10) and idic(8;?)(p10;?) present in hypertetraploid clone |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |