微信小李
微信小章
产品简介
购买须知
| 产品名称： | CAL-12T |
|---|---|
| 商品货号： | TS259632 |
| <strong>Cell line </strong>： | CAL-12T |
| DSMZ no. ： | TS259632 |
| Species ： | originally confirmed as human with IEF of AST, NP and recently by STR-fingerprinting |
| Cell type ： | non-small cell lung carcinoma |
| Origin ： | established from a male patient with a non-small cell lung carcinoma in 1981 |
| Reference(s) ： | 14825, 14535 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 ; split confluent culture 1:2 to 1:3 every second day using trypsin/EDTA (incubation with trypsin/EDTA for about 15-20 min) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 59(57-59)<3n>X, +1, -2, -4, -5, -6, -6, -7, -11, -12, -13, -16, -16, -18, +20, +4-5mar, der(1;18)(p10;q10), der(1)t(X;1)(?;p?21)t(1;?)(q1?1;?), der(1)(q12->q10::p10->pter), add(3)(p11), add(4)(p11), add(5)(p15), add(9)(q1?)x1-2, add(11)(q24), dup(11)(q13q24), del(12)(p12), i(14q), add(19)(q11), i(21q) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |