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| 产品名称： | MS-5 |
|---|---|
| 商品货号： | TS259634 |
| <strong>Cell line </strong>： | MS-5 |
| DSMZ no. ： | TS259634 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | stromal cells |
| Origin ： | established by irradiation of the adherent cells in long-term bone marrow cultures derived from C3H/HeNSlc strain mice |
| Reference(s) ： | 14633 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastic cells growing in monolayers; image; image; image |
| Medium ： | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% horse serum (or h.i. FBS) + 2 mM L-glutamine + 2 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every third day using trypsin/EDTA; maximal density at ca. 15 x 106 cells/175 cm2; CAVEAT: cells may detach spontaneously in aggregates which are difficult to disperse |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype |
| Viruses ： | PCR: SMRV - |