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| 产品名称： | FU-OV-1 |
|---|---|
| 商品货号： | TS259636 |
| <strong>Cell line </strong>： | FU-OV-1 |
| DSMZ no. ： | TS259636 |
| Species ： | human (Homo sapiens) |
| Cell type ： | ovarian carcinoma |
| Origin ： | established post-hysterectomy from the tumor tissue of a 65-year-old Japanese woman with malignant ovarian carcinoma (stage: FIGO IIIc; histology: poorly differentiated serous papillary adenocarcinoma) in 1989 |
| Reference(s) ： | 14510 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers; image; image |
| Medium ： | 80-90% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 120 hours |
| Harvest ： | cell harvest of ca. 5-10 x 106 cells/175 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 12% polyploidy - 58-62<3n>X, -X, -X, -1, -2, -3, -4, -7, +9, -10, -12, -13, -14, -16, -17, -17, -18, -19, -20, -20, -22, +fra, +dm, del(2)(p23), inv(3)(p25q29), del(4)(q21/22), add(5)(p11), add(6)(q27), add(9)(p11), add(11)(p15)x2, add(11)(q24), add(14)(p11), add(14)(p11), add(16)(p13), add(19)(q13)x2, add(?21)(p11)x2 - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |