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| 产品名称： | 22RV1 |
|---|---|
| 商品货号： | TS259637 |
| <strong>Cell line </strong>： | 22RV1 |
| DSMZ no. ： | TS259637 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | prostate carcinoma |
| Origin ： | cell line was derived from a human prostate carcinoma xenograft (CWR22R) that was serially propagated in nude mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft; the cells contain proviruses of the gammaretrovirus XMRV and this cell line has been assigned by the ZKBS (German Central Commission for Biological Safety) to risk category 2 |
| Reference(s) ： | 14979, 15048 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line was shown to carry more than 10 copies of the xenotropic murine leukemia virus related virus integrated as proviruses into the eukaryotic genome. Active viruses are secreted into the cell culture supernatant. Murine retroviruses can cause leukemia, lymphoma or other tumors. A relation to analogous diseases in human could not be shown. Thus, the viruses and the cell line was categorized bio safety level 1. However, the German Commission for Biosafety (ZKBS) classified this cell line risk group 2 when the cells are used for genetic manipulations. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers; image |
| Medium ： | 40% RPMI 1640 + 40% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells in a 175 cm2 flask; split confluent culture 1:3 to 1:10 every 3-6 days using trypsin/EDTA for ca. 10 min |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175cm 2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 50(48-52)<2n>XY, +1, +7, +8, +12, i(1q), der(2)t(2;4)(p1?4;q3?3)del(2)(q11q3?1), der(4)t(2;4)(p1?4;q3?3), add(6)(q15), add(14)(q24) - sideline with del(1)(q21), +3, +18 - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV -, XMRV + |