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| 产品名称： | CAL-62 |
|---|---|
| 商品货号： | TS259642 |
| <strong>Cell line </strong>： | CAL-62 |
| DSMZ no. ： | TS259642 |
| Species ： | human (Homo sapiens) |
| Cell type ： | thyroid anaplastic carcinoma |
| Origin ： | established from the thyroid gland (right lobe) of a 70-year-old woman with thyroid anaplastic carcinoma in 1988; described as being tumorigenic in heterotransplanted nude mice |
| Reference(s) ： | 14564 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in monolayers; image |
| Medium ： | 90-95% Dulbeccos MEM + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:10 to 1:15 every 3-5 days using trypsin/EDTA; maximal density at ca. 14 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 28 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human, flat-moded hypertriploid karyotype with 8% polyploidy - 60-76<3n>XX, -X, +3, +3, -4, +6, +7, +9, -11, -14, -18, +19, +20, +20, +2-6mar - del(1)(p34), der(1)t(1;17)(p34;q21), der(3)add(3)(p11)?dup(3)(q21q22)x2-4, del(6)(q15)x2, i(8q), der(?12)t(1;12)(q11-12;q14), add(17)(q21), add(21)(q22) - sideline with del(X)(q24), der(7;8)(q10;q10) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |