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| 产品名称： | CAL-27 |
|---|---|
| 商品货号： | TS259644 |
| <strong>Cell line </strong>： | CAL-27 |
| DSMZ no. ： | TS259644 |
| Species ： | confirmed as human by STR-typing |
| Cell type ： | tongue squamous cell carcinoma |
| Origin ： | established from the poorly differentiated squamous cell carcinoma of the tongue removed from a 56-year-old man before treatment in 1982 |
| Reference(s) ： | 14562 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayer; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out 1-2 x 106 cells in a small (25cm2) culture flask; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; maximum density of ca. 14-18 x 106 cells/80cm2 flask |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 20-40 hours |
| Harvest ： | cell harvest of ca. 8-12 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 3% polyploidy - 41(38-45)<2n>X, -Y, -4, +7, -10, -11, -18, -20, -21, -22, +2-3mar, add(3)(q21), i(3q), i(7p), del(8)(p21), i(9q), add(13)(p11), add(14)(p11) - sideline with additional der(3) and der(7) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |