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| 产品名称： | HKT-1097 |
|---|---|
| 商品货号： | TS259647 |
| <strong>Cell line </strong>： | HKT-1097 |
| DSMZ no. ： | TS259647 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin confirmed by COI DNA barcoding |
| Cell type ： | kidney tumor |
| Origin ： | established from diethylstilbesterol-induced renal tumors of a 14-month-old male Syrian Golden Hamster in 1997; cells were described as expressing estrogen receptors, glial fibrillary acidic protein and other markers of the neuroectodermal lineage and as being responsive to estrogen |
| Reference(s) ： | 14408, 14708, 14922 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid cells growing in monolayers |
| Medium ： | 95% Dulbeccos MEM (4.5 g/L glucose) + 5% h.i. FBS + 2 mM L-glutamine + 25 mM HEPES |
| Subculture ： | split confluent culture 1:20 to 1:50 every 3-4 days using trypsin/EDTA; seed out at ca. 0.05 x 106 cells/cm2; maintain at 0.2 x 106 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 19 hours |
| Harvest ： | cell harvest of ca. 0.3 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hyperdiploid hamster karyotype with 10% polyploidy - s = 45(39-46) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, SMRV - |