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| 产品名称： | RO |
|---|---|
| 商品货号： | TS259649 |
| <strong>Cell line </strong>： | RO |
| DSMZ no. ： | TS259649 |
| Species ： | human (Homo sapiens) |
| Cell type ： | B lymphoblastoid cells (from patient with severe combined immunodeficiency, SCID) |
| Origin ： | established from the peripheral blood of a 3-year-old boy with severe combined immunodeficiency (SCID) by EBV-transformation using the cell line B95-8 as source for EBV; cells were described as lacking MHC class II molecules |
| Reference(s) ： | 14453, 14983, 15172, 15173 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in suspension, singly or in small clusters |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split saturated culture at 1:2 every two days; seed out at ca. 1.0 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates; cell growth may be slow for the first week maximal density at ca. 2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR -, sm/cyIgG (5-10%)+, sm/cyIgM (20-40%)+, sm/cykappa (5-10%)+, sm/cylambda (10-20%) |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 8% polyploidy - 46(43-46)<2n>XY, no consistent abnormality present |
| Viruses ： | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |