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| 产品名称： | WMP-2 |
|---|---|
| 商品货号： | TS259651 |
| <strong>Cell line </strong>： | WMP-2 |
| DSMZ no. ： | TS259651 |
| Species ： | confirmed as mus musculus by COI DNA barcoding |
| Cell type ： | lymphoblasts |
| Origin ： | established from spleen and lymph nodes of an adult Maloney murine leukemia virus (MoMuLV)-infected female WMP/WMP wild mouse; cells are described in the literature to carry nine pairs of metacentric Robertsonian translocation chromosomes containing 1 to 18; cells can be used for physical gene mapping by in situ hybridization in mice |
| Reference(s) ： | 15079 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 50 µM mercaptoethanol |
| Subculture ： | split saturated culture at 1:3 to 1:5 every 2-4 days; seed out at ca. 2.5 x 105 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 2.0-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine karyotype with a preponderance of centric fusion markers - resembles published karyotype |
| Viruses ： | PCR: SMRV - |