微信小李
微信小章
产品简介
购买须知
| 产品名称： | RED-5 |
|---|---|
| 商品货号： | TS259653 |
| <strong>Cell line </strong>： | RED-5 |
| DSMZ no. ： | TS259653 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described as being responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture at 1:2 to 1:3 every two days; seed out at ca. 0.5-1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover subsequently over the next week and viability will raise over the next few passages (spent medium should be exchanged without discarding cells); cells grow initially better in 24-well-plates; maximal density at ca. 2.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine-like karyotype |
| Viruses ： | PCR: SMRV - |