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| 产品名称： | BC-3C |
|---|---|
| 商品货号： | TS259656 |
| <strong>Cell line </strong>： | BC-3C |
| DSMZ no. ： | TS259656 |
| Species ： | human (Homo sapiens) |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | derived from the invasive solid transitional cell carcinoma of the bladder of a 82-year-old Caucasian woman (grade IV, stage C); cell growth described as being inhibited by EGF |
| Reference(s) ： | 14857 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing adherently in monolayers and in multilayer foci; image; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:10 every 3-7 days using trypsin/EDTA; seed out at ca. 0.25-0.4 x 105 cells/cm2; recover and initially culture cells in 20% FBS; maximum density of ca. 3.5 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25-30 hours |
| Harvest ： | cell harvest of ca. 2-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 10% polyploidy - 65-72<3n>XX, -X, -2, -4, +9, +9, -11, -13, +14, +16, -17, -17, -18, +19, +20, +20, +2-3mar, add(2)(p1?), del(3)(q2?2), der(5;7)(q10;q10), del(9)(p2?2p2?3), add(12)(q24) - sideline with del(2)(q2?), i(3q), add(7)(p22), add(8)(p12), add(11)(q22-24), i(?17p), i(17q) and extra numerical changes - matches published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |