微信小李
微信小章
产品简介
购买须知
| 产品名称： | CAL-78 |
|---|---|
| 商品货号： | TS259657 |
| <strong>Cell line </strong>： | CAL-78 |
| DSMZ no. ： | TS259657 |
| Species ： | human (Homo sapiens) |
| Cell type ： | chondrosarcoma |
| Origin ： | established from the recurrence of a dedifferentiated chondrosarcoma (grade III) of the muscle of the thigh from a 76-year-old man in 1989; tumorigenic chondrosarcomatous differentiation of xenografted tumor |
| Reference(s) ： | 15150 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblast-like cells growing as monolayer; the culture contains a large amount of cellular debris; image ; image ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; cells grow extremely slowly; split confluent culture 1:2 every 10-12 days using trypsin without EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 240 hours |
| Harvest ： | cell harvest of ca. 10-14 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |