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| 产品名称： | AC-1M59 |
|---|---|
| 商品货号： | TS259658 |
| <strong>Cell line </strong>： | AC-1M59 |
| DSMZ no. ： | TS259658 |
| Species ： | human (Homo sapiens) |
| Cell type ： | choriocarcinoma-trophoblast hybrid |
| Origin ： | subclone of ACH1P; ACH1P was established by somatic hybridization of primary male trophoblast cells and the HGPRT-defective choriocarcinoma cell line AC1-1 which was originally derived from cell line JEG-3 (DSM ACC 463); in contrast to the respective sister cell lines, AC-1M59 was described as expressing ERK5, cytokeratin I, TNF-R2, VEGFR-2 and IL-2 |
| Reference(s) ： | 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:4 every 3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 8% polyploidy - 86-97<4n>XXXY, -4, +7, -8, +9, -13, -13, -18, +19, +2-5mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32), add(9)(p24), add(11)(p15), i(13q), add(15)(p13), add(19)(p13)x1-2 - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) - the DNA fingerprint finding of 3 alleles at loci D2S44 and D17S5 suggests additional human chromosomes |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |