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| 产品名称： | CAL-120 |
|---|---|
| 商品货号： | TS259660 |
| <strong>Cell line </strong>： | CAL-120 |
| DSMZ no. ： | TS259660 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast adenocarcinoma |
| Origin ： | established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers; image; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture 1:2 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 6-8 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), del(4)(p15.2), add(6)(q11)x2, del(7)(q32), add(8)(p12), add(8)(q24), der(8;13)(q10;q10), ins(11;?)(q13;?), add(12)(p1?), add(13)(p11)x1-2, add(14)(p11), der(15;?21)(p1?1;?q12)x3 - sideline with i(1)(p10)add(1)(p3?2), i(1q), i(3p), der(16)t(5;16)(q13;q24) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |