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| 产品名称： | AC-1M81 |
|---|---|
| 商品货号： | TS259664 |
| <strong>Cell line </strong>： | AC-1M81 |
| DSMZ no. ： | TS259664 |
| Species ： | human (Homo sapiens) |
| Cell type ： | choriocarcinoma-trophoblast hybrid |
| Origin ： | subclone of ACH1P which was established by fusion of the JEG-3-derived HGPRT-defective mutant cell line AC1-1 with primary male trophoblast cells isolated from term human chorion laeve; described as possessing both, a distinct karyotype and a distinct expression pattern of tumor-related genes |
| Reference(s) ： | 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 0.4 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with sparfloxacin, then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | near identical DNA profiles shared by the AC-sister cell lines, partly identical with JEG-3 and BEWO |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 4% polyploidy - 80-106<4n>XX, -Y, -3, -4, +7, +9, -13, -18, +22, +4-6mar, del(X)(p11p22), der(X)(Xpter->Xq2?8::4p1?->4p1?::11q1?1->11qter), der(7)t(7;7)(p22;q11.22)add(7)(q32), add(7)(q32)x2, i(13q), add(15)(p13), add(19)(p13), der(19)t(11;19)(q11;q13) - resembles published karyotype and those of sisters with minor, though consistent, differences (mirroring DNA profiles) |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |