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| 产品名称： | JEG-3 |
|---|---|
| 商品货号： | TS259669 |
| <strong>Cell line </strong>： | JEG-3 |
| DSMZ no. ： | TS259669 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | choriocarcinoma |
| Origin ： | established from a human gestational choriocarcinoma which was taken at autopsy from a cerebral metastasis; cells were initially propagated in hamster cheek pouches by serial passage prior to cultivation in culture; serial sister cell line of cell line BEWO (DSM ACC 458) |
| Reference(s) ： | 14682, 14836, 14543 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer and carrying many intracellular dark granula; image |
| Medium ： | 90% Hams F12 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm2; maintain at ca. 10 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | cell harvest of ca. 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as BEWO using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 12% polyploidy - 71(69-72)<3n>XXY, +2, +5, +9, -11, -13, -18, +4-6mar, ?del(X)(p11.4), der(4)t(4;11)(p16;q13), add(6)(q2?7)x1-2, add(7)(q32), add(7)(p22), i(13q), add(15)(p11), add(19)(p13) - sideline with add(8)(p1?), add(10)(q21), ?del(18)(q22) - resembles published karyotype and (marginally) that of BEWO |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |