微信小李
微信小章
产品简介
购买须知
| 产品名称： | JAR |
|---|---|
| 商品货号： | TS259672 |
| <strong>Cell line </strong>： | JAR |
| DSMZ no. ： | TS259672 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | choriocarcinoma |
| Origin ： | established from the trophoblastic tumor of the placenta of a 24-year-old Caucasian woman, but the cell line is from the male fetus |
| Reference(s) ： | 15154 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherent in monolayers and in clusters |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | split culture 1:5 to 1:10 every 3-4 days before reaching confluency using trypsin/EDTA; seed out at ca. 3-4 x 106 cells/80 cm2; confluent cultures tend to form aggregates, that loosely adhere to the culture vessel surface; however, these aggregates are difficult to disperse and may need trypsin treatment after a centrifugation and washing step |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 20-30 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near-triploid karyotype with 27% polyploidy - 60-70<3n>XXY, +1, -2, -5, -7, -8, -9, -13, -13, -14, -15, -17, -19, -21,+12mar, +acf, add(X)(p22), del(1)(p3?p3?), add(1)(q11), der(1)add(1)(p11)add(1)(q4?), inv(3)(p25.2q24), add(3)(q13), add(4)(q?31), del(4)(q2?2), add(9)(p21), add(12)(q24), i(13)(q10)dup(13)(q11q34), add(16)(q24), add(17)(p12), add(18)(p11.2) - sideline with add(2)(p2?), del(8)(q22), add(21)(p11) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |