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| 产品名称： | CAL-148 |
|---|---|
| 商品货号： | TS259673 |
| <strong>Cell line </strong>： | CAL-148 |
| DSMZ no. ： | TS259673 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | breast adenocarcinoma |
| Origin ： | established from the pleural effusion from a 58-year-old French woman with terminally invasive breast cancer (moderately differentiated ductal adenocarcinoma) after repeated cycles of chemotherapy (including adriamycin and taxol) |
| Reference(s) ： | 14902 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round to epitheloid cells growing as single cells or as clumps either in suspension or, if supplemented with EGF, in monolayers; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS + 2 mM L-glutamine + EGF (1 µg/100 ml, optional) |
| Subculture ： | seed out at ca. 0.2-0.5 x 106 cells/ml (suspension culture) or 0.4-0.8 x 105 cells/cm2 (adherent culture); maintain at about 0.2-1.5 x 106 cells/ml or equivalent; split confluent culture 1:4 to 1:8 every 3-5 days by agitation; cell number may be difficult to ascertain due to growth in clumps; cells grow faster in suspension without EGF, however EGF supports monolayer formation |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/ml or 1 x106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 16% polyploidy - 45-48<2n>XX, +1, +7, -8, -13, +20, add(1)(p36), der(1;16)(q10;p10), del(2)(q11q2?2), del(13)(q1?1q1?3), der(16)t(1;16)(q12-21 - p11) - resembles originators unpublished karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |