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| 产品名称： | ESS-1 |
|---|---|
| 商品货号： | TS259674 |
| <strong>Cell line </strong>： | ESS-1 |
| DSMZ no. ： | TS259674 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | endometrial stromal sarcoma |
| Origin ： | established from an endometrial stromal sarcoma of the uterus of a 76-year-old Caucasian woman; cells were described as demonstrating a mainly mesenchymal phenotype with signs of epithelial characteristics within foci and an extensively multilayered growth pattern |
| Reference(s) ： | 14582, 14546 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers, partially also in multilayers; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 every week using trypsin/EDTA, but change the medium more frequently; maximum density of ca. 10-15 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 120 hours |
| Harvest ： | cell harvest of ca.10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human karyotype with hyper-diploid/tetraploid and -octaploid clones present in approx. equal numbers - 47(45-47)<2n>XX, +19, der(3)t(3;6)(q2?8;p11-12), add(6)(q27), add(19)(p11) - sideline with i(2q) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |