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| 产品名称： | ML-1 |
|---|---|
| 商品货号： | TS259676 |
| <strong>Cell line </strong>： | ML-1 |
| DSMZ no. ： | TS259676 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | follicular thyroid carcinoma |
| Origin ： | established from a recurrent tumor of a dedifferentiated follicular thyroid carcinoma (stage pT4) of a 50-year-old woman; cells were described as taking up iodine and/or glucose and as secreting thyroglobulin, chondroitin sulfate, fibronectin, and triiodothyronine; cells are tumorigenic in nude mice |
| Reference(s) ： | 14945 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers; image; image; image |
| Medium ： | 80-90% Dulbeccos MEM (4.5 g/L glucose) + 10-20% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | split confluent culture 1:2 once a week using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 4-7 days |
| Harvest ： | cell harvest of ca. 25-30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human extreme-hypodiploid karyotype with 12% polyploidy - 37(33-38)<2n>XX, -6, -13, -14, -17, -18, -19, -21, add(4)(p16), add(9)(q22)x1-2, der(19)t(9;19)(q1?2;p1?2)/der(9;19)(q10;q10)x1-2 - sideline with idem, der(3;19)(?q10;?q10), -11 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |