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| 产品名称： | CL-11 |
|---|---|
| 商品货号： | TS259679 |
| <strong>Cell line </strong>： | CL-11 |
| DSMZ no. ： | TS259679 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | colon carcinoma |
| Origin ： | established from the primary tumor at the left colon (a moderately differentiated colorectal carcinoma of TNM stage 3); sister cell line of mucoid growing cell line CL-11M; cells were described as expressing tumor-associated carcino-embryonic antigen (CEA) |
| Reference(s) ： | 14528 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers and forming islets; image; image; image |
| Medium ： | 80% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:5 once or twice a week using trypsin/EDTA; maximal density at ca. 1.5 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 3-4 days |
| Harvest ： | cell harvest of ca. 12 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 12% polyploidy - 46(45-47)<2n>XY, -6, +7, -17, -18, +2-3 mar, +1-4 supernumerary r/dm, add(8)(q24) - sideline with idem, +7 |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |