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| 产品名称： | SIG-M5 |
|---|---|
| 商品货号： | TS259681 |
| <strong>Cell line </strong>： | SIG-M5 |
| DSMZ no. ： | TS259681 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | acute monocytic leukemia |
| Origin ： | established in 1995 from the bone marrow of a 63-year-old man with acute myeloid leukemia of monocytic origin (AML FAB M5a) at diagnosis |
| Reference(s) ： | 15049 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large round cells growing singly in suspension; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS |
| Subculture ： | cells may be difficult to culture; seed out at ca. 1 x 106 cells/ml; split saturated culture 1:2 every 3 days; after thawing, it is advisable to initially grow the cells in 12- or 24-well plates; cell viability remains continuously at or below 70% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | maximum density of about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid/hypotetraploid karyotype with 4% polyploidy - 78(77-81)<3n>XX/XXY, +1, +2, +3, +4, +5, +8, -9, -10, +12, +15, +16, +17, +18, +21, +22, +0-2mar - i(1q), add(17)(p1?1) - resembles published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |