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| 产品名称： | GH3 |
|---|---|
| 商品货号： | TS259682 |
| <strong>Cell line </strong>： | GH3 |
| DSMZ no. ： | TS259682 |
| Species ： | originally confirmed as rat by cytogenetics and species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | pituitary tumor |
| Origin ： | derived in 1965 from a pituitary tumor induced in a female Wistar/Furth rat following repeated transplantation with the MtT/W5 tumor (originally induced in a female F/Fu rat by cranial X-irradiation) |
| Reference(s) ： | 15004, 15008 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in loosely adherent in semi-confluent monolayers and as cell clusters in suspension; image |
| Medium ： | 82.5% Hams F10 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:5 every 2-5 days, cells may be detached using trypsin/EDTA or mechanically by agitation; seed out at ca. 4 x 106 cells/80 cm2; maintain at 0.3-0.5 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60-90 hours |
| Harvest ： | maximal density of 2 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | hypertriploid rat karyotype with 20% poylpoidy - 68-71<3n> |
| Viruses ： | PCR: SMRV - |