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| 产品名称： | BAG-12G2 |
|---|---|
| 商品货号： | TS259684 |
| <strong>Cell line </strong>： | BAG-12G2 |
| DSMZ no. ： | TS259684 |
| Species ： | confirmed as mouse by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CDw78) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2akappa) CDw78; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells |
| Reference(s) ： | 15169 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension (some cells adhere partly) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") |
| Subculture ： | split saturated cultures 1:2 to 1:4 every 1-3 days; seed out at 0.25 x 106 cell/ml; maintain at 0.1-0.8 x 106 cells/ml; cells may grow slowly after initial recovery from N2 |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | maximal density of about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2a monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |