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| 产品名称： | PC-3 |
|---|---|
| 商品货号： | TS259686 |
| <strong>Cell line </strong>： | PC-3 |
| DSMZ no. ： | TS259686 |
| Species ： | confirmed as human by cytogenetics |
| Cell type ： | prostate carcinoma |
| Origin ： | established from the bone marrow metastasis isolated post-mortem from a 62-year-old Caucasian man with grade IV prostate cancer (poorly differentiated adenocarcinoma) after androgen suppression therapy; described in the literature to form tumors in nude mice, to grow in soft agar, and to be unresponsive to androgen treatment |
| Reference(s) ： | 14644, 14832, 15188 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing adherently in monolayers or in multilayer foci |
| Medium ： | 45% Hams F12 + 45% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of 3-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly-rearranged flat-moded hypotetraploid karyotype with 3% polyploidy - 80-90<4n>XX/XXX, -Y, -1, -1, -2, -2, +3, +3, -4, -5, +7, -8, -8, -10, -10, -10, -10, -12, -13, +14, -15, -15, -16, -16, -17, -18, -18, -22, +18-21mar, +1-2dm, del(1)(p34p35), add(1p?32), add(3)(p1?), add(3)(q13), add(3)(q21)x2, del(5)(p1?)x1-2, add(9)(p1?1), del(9)(p21)x1-2, add(12)(q24), add(13)(p11), del(17)(p11), add(19)(p13) - sideline with der(3)add(3)(p2?5)add(3q2?), del(4)(q2?4q2?8), add(7)(q11), add(8)(p1?), add(9)(q34) - extensive genomic amplification of c-MYC in hsr present in markers - distantly related to published karyotype |
| Viruses ： | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |