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| 产品名称： | CAT-13.1E10 |
|---|---|
| 商品货号： | TS259689 |
| <strong>Cell line </strong>： | CAT-13.1E10 |
| DSMZ no. ： | TS259689 |
| Species ： | origin as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (CD19) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD19; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with EBV-transformed B cells from a patient with MHC class-II deficiency re-expressing class-II antigens |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing as monolayers, some grow in suspension |
| Medium ： | 80% Dulbeccos MEM (4.5 g/L glucose) + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated cultures 1:3 every 2 days; seed out at 0.2 x 106 cell/ml; maintain at ca. 0.2-0.4 x 106 cells/ml; after thawing cells may start slowly to take up growth |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | maximal density of about 5 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with sparfloxacin, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |