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| 产品名称： | CAT-13.0B10 |
|---|---|
| 商品货号： | TS259694 |
| <strong>Cell line </strong>： | CAT-13.0B10 |
| DSMZ no. ： | TS259694 |
| Species ： | mus musculus origin by COI DNA Barcoding |
| Cell type ： | hybridoma (CD39) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) CD39; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with EBV-transformed B cells from a patient with MHC class-II deficiency re-expressing class-II antigens |
| Reference(s) ： | 15167 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension and some slightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement" for the first 1-2 weeks post-thawing) |
| Subculture ： | split saturated culture 1:2 to 1:4 every 2 days; seed out at 0.3 x 106 cells/ml; maintain at 0.2-0.4 x 106 cells/ml; best to culture cells for the first 5-7 days in a 12-well-plate with HFCS, then cells may be transfered to flasks; after 14 days, cells become slightly adherent (about two-thirds of them), but can be detached by slapping the flask; the HFCS concentration can be decreased after the initial 2 weeks to about 50% (1:100) or 25% (1:200) |
| Incubation ： | at 37°C with 10% CO2 |
| Doubling time ： | about 25 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 in 30 ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |